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Piedmontese Only
What is the "coat color test?"
The coat color test identifies individuals that carry the gene for recessive red coat color (red factor). This test identifies the actual mutations in the gene that causes expression of black or red coat color. The test identifies the genotype of an animal as Homozygous Black, Red Carrier, or carriers of the Wildtype allele (animals that carry an allele associated with the "Red-Black" condition in Holstein cattle).

Do we have to have a vet draw the blood, or can we draw it ourselves?
The customers may draw the blood themselves by following the instructions provided with the kit or have a licensed veterinarian draw the blood.

Does the animal have to be a certain age to be tested?
There is no age requirement. Samples may be collected as soon as the animal is born. However, if pulling hair samples we recommend that you wait approximately 24 hours after birth of the animal in order to allow the hair follicles to fully develop.

Is there a difference in the accuracy of DNA testing whether hair roots or blood samples are submitted?
No, the accuracy of DNA testing is the same.

Are test results forwarded to the breed association?
Test results for coat color on Holstein Cattle and Limousin Cattle are automatically forwarded to the respective breed association. Test results for BLAD status on Holstein Cattle are also forwarded to the breed association.

Does GenMARK offer commercial Polled testing?
No, research on the genes involved in the polled trait is still in progress. At this time, we cannot offer a commercial test for Polled.

Can the coat color test identify whether or not an animal carries the Diluter gene?
No, the coat color test cannot provide any information regarding the presence of the Diluter gene. At this time little is known regarding the actual DNA mutations that cause the expression of Diluter.

What do my coat color test results mean?
  • Homozygous Black - this animal carries two copies of the dominant black allele for coat color and will only produce animals black in color. However, the black offspring could be red carriers if the animal is mated to a red carrier or red animal.
  • Red Carrier - this animal carries one copy of the dominant black allele and one copy of the recessive red allele (red factor). This animal will be black in color but will pass along the recessive red allele to its offspring 50% of the time and could produce either black or red offspring depending on the mating.
  • Heterozygous Black / Wildtype - this animal carries one copy of the dominant black allele and one copy of the wildtype allele. In beef breeds, this animal will most often be black in color but will pass along the wildtype allele to its offspring 50% of the time. The wildtype allele is associated with the Red-Black condition in Holstein cattle but has been poorly characterized in beef cattle. It has been reported that animals carrying the wildtype allele occasionally appear red when born and gradually turn black as they age. Additionally, some animals carrying the wildtype allele have been reported to have a reddish color at the base of the hair and black along the remainder of the hair shaft.
  • Heterozygous Red / Wildtype - this animal carries one copy of the recessive red allele and one copy of the wildtype allele. The coloring of these animals is variable and has been poorly characterized, especially in beef cattle. Most often, this animal will appear red at birth but may gradually darken or turn black as it ages.
  • Homozygous Red - this animal carries two copies of the recessive red allele. This animal will be red in color (also known as "true red") and will always pass on the recessive red allele to its offspring. However, this animal can produce offspring either red or black in color, depending upon the mating

How can I select for Coat Color in my herd?
As a producer, you can use the GenMARK coat color test results to help you increase the incidence of Homozygous Black or Homozygous Red in your herd, depending on your desires. Consider these possible mating schemes and their outcomes for managing Coat Color.

Mating a Homozygous Black animal to a Homozygous Black animal will always produce Homozygous Black offspring and will eliminate the need to DNA test future offspring.

Mating a Homozygous Black animal to a Red Carrier will produce:

  • 50% Homozygous Black offspring
  • 50% Red Carrier offspring
Mating a Homozygous Black animal to a Homozygous Red animal will always produce Red Carrier offspring

Mating a Red Carrier animal to a Red Carrier animal will produce:

  • 25% Homozygous Black offspring
  • 50% Red Carrier offspring
  • 25% Homozygous Red animals
Mating a Homozygous Red animal to a Homozygous Red animal will always produce Homozygous Red offspring

If you are a beef breeder who has wildtype in your herd and are interested in breeding Homozygous Black animals, simply treat your Heterozygous Black / Wildtype animals as Red Carriers in the above mating schemes.


Do I need to test my red animals for Coat Color?
If you have a red animal, there is usually no need to perform coat color testing. However, if you are a breeder who is trying to breed Homozygous Red animals and have found that you carry the wildtype allele in your herd, you may want to test the animal to ensure that it is Homozygous Red, not Heterozygous Red / Wildtype.

How does DNA typing for parentage differ from the previous system of blood typing?
Converting from blood typing to DNA typing for parentage and pedigree validation may appear at first to be a hassle as many previously typed sires and dams must be resubmitted for DNA analysis. DNA typing, however, is much more useful for pedigree validation as it can be performed using samples from animals that are no longer available. Blood typing requires fresh whole blood for analysis whereas hair roots, semen or tissue can be submitted for DNA typing.

How accurate is DNA typing for parentage?
The use of microsatellite DNA for parentage verification provides a level of exclusion for any parent of up to 99%.

What is a microsatellite?
Microsatellite sequence consists of repeated units of the genetic code G, A, T or C; the most common repeat unit is CA. For example, a particular microsatellite may consist of 10 repeated CA units. During evolution, a mutation in the DNA may result in some individuals having 8 repeat units whereas others may have 6 or 12 repeats at that same locus. At any one microsatellite locus, three to ten or more possible variations may exist in the population. Approximately 3000 microsatellite loci have been identified by laboratories around the world and placed on the Bovine Genome Map. For parentage analysis, a standardized and highly informative panel of microsatellite markers have been approved by the International Society of Animal Genetics (ISAG).

Are blood types transferable to DNA types?
No, blood types cannot be converted to DNA types. A blood type represents a protein that is encoded by a gene. DNA types identify specific variations in DNA sequences found in the non-coding regions throughout an animal's genome.

What is the difference between a "DNA Profile" and "Parentage?
" Parentage testing determines whether a sire or dam qualifies as an individual's parent. DNA extracted from hair roots, blood or semen is analyzed with a battery of eleven standardized DNA microsatellite markers to produce a unique DNA Profile or "fingerprint." This profile remains as a permanent record of the individual's genetic identity. Parentage analysis entails comparing the DNA Profiles of an individual with its assumed parents to detect any discrepancies that may cause one or more parents to be excluded.

Do we need to send in both the sire and dam when parentage testing?
If possible, however parentage analysis can still be performed if DNA on only one parent is available. If the sire or dam were DNA typed previously, then their DNA Profiles can be used for parentage analysis of any offspring. If you have questions as to whether to submit both sire and dam for parentage, please call PAUS at 1-262-920-7287.

What if the animal is no longer alive?
If the sire is no longer alive but semen was collected, then one straw of semen can be submitted and will provide enough DNA for a multitude of tests. If the dam is dead, then it will be impossible to conduct DNA testing unless hair roots or whole blood were collected previously and stored.

Does semen need to be shipped frozen?
No, a straw of semen can be thawed to room temperature before shipment. To ship semen for DNA testing, place straw(s) in a padded envelope and send by U.S. Mail. One straw of semen provides enough DNA for several tests.

How will results of parentage testing be reported?
Parentage testing is performed at the beginning of each month. Parentage results will be reported during the second week of each month. The result report will indicate whether the parent in question Qualifies or Does Not Qualify as sire or dam of an individual. A copy of this report will also be forwarded to the appropriate PAUS offices.

Why do I have to pay to have a sire or dam typed again if these animals have already been blood typed by Stormont?
Blood typing results cannot be converted to DNA types; therefore an individual must have DNA submitted for analysis and permanent record in the PAUS database. Specifically, a blood type represents a protein that is encoded by a gene. DNA types identify specific variations in DNA sequences found in the non-coding regions throughout an animal's genome.

Why test for myostatin?
The DNA test for Myostatin identifies the point mutation that renders the myostatin gene inactive and causes the "double-muscling" phenotype of Piedmontese cattle. Full-blood Piedmontese cattle have two copies of the inactive myostatin gene and are often referred to as "Two Copy" individuals. Animals that have only one copy of inactive myostatin look normal but produce meatier carcasses with less fat than individuals with the normal myostatin gene.

How will myostatin results be reported?
Test results of individuals that possess two copies of the Piedmontese myostatin mutation will be reported as Pied-Two Copy. Individuals with one copy of the mutation will be reported as Pied-One Copy. Likewise, individuals that possess two copies of the normal myostatin gene will be identified as Pied-Zero Copy.

What are the approximate turn-around times for myostatin and parentage test results?
Parentage and myostatin testing are performed on a monthly basis. Results for these tests will be available during the second week of each month. Reporting of test results will be delayed if payment is missing or the Genetic Disclaimer is not signed.

How do I submit samples for DNA testing?
Blood samples and hair roots can be submitted for diagnostic DNA testing. Forms needed for submitting samples may be downloaded from this site. Hair samples may be submitted with the corresponding paperwork in individually sealed plastic sandwich bags or in individually sealed envelopes - please be sure to clearly label each sample with the corresponding animal ID. Please limit one sample per bagor per envelope. When submitting semen for parentage, please use a padded envelope, with the semen between two pieces of cardboard. This will prevent the semen straw from bending during shipping. Semen should be shipped at room temperature.

What needs to be included when samples are sent to the lab?
For orders by mail, please include:
One sample of hair roots (or one straw of semen) for each animal to be tested.

Signed Genetic Disclaimer.
Registration information for each animal or registration numbers of the sire and the dam.
Completed Request for Parentage and Myostatin Genotyping.
Payment - Please make checks payable to GenMARK, or fill out the credit card information located on the pricing sheet and submit payment with your sample(s).


Does the animal have to be a certain age to be tested?
There is no age requirement. Samples may be collected as soon as the animal is born. However, if pulling hair samples we recommend that you wait approximately 24 hours after birth of the animal in order to allow the hair follicles to fully develop.

Are test results forwarded to the breed association?
Parentage and myostatin test results will be reported to the registration office:
PAUS
Attn: Beth Lavy
343 Barrett Road
Elsberrt, MO 63343-4137
Phone: 573-384-5685
Fax: 573-384-5567
Email: paus343@yahoo.com